Description

Sidonia B.G. Eckle, Jamie Rossjohn, and James McCluskey

2. Antibodies in Transplantation: The Effects of HLA and Non-HLA Antibody
Binding and Mechanisms of Injury

Nicole M. Valenzuela and Elaine F. Reed

3. Cell Mediated Rejection

Kathryn J Wood, Masaaki Zaitsu and Ryoichi Goto

4. Transplantation Tolerance

Colin Brinkman, Bryna Burrell, Joseph Scalea, and Jonathan Bromberg

5. Composite Tissue Transplantation

Gerald Brandacher

6. Transplantation of the Sensitized Patient: Histocompatibility Testing

Robert A. Montgomery, Mary S. Leffell, and Andrea A. Zachary

7. Thoracic Organ Transplantation: Laboratory Methods

Jignesh K. Patel and Jon A. Kobashigawa

PART II: Methods Chapters

8. HLA Typing by Sequence Specific Primers

Mike Bunce, Biofortuna and Ben Passey, Biofortuna

9. Human Leukocyte Antigen (HLA) Typing By DNA Sequencing

Ana Lazaro, Bin Tu, Ruyan Yang, Yi Xiao, Kanthi Kariyawasam, Jennifer Ng, Carolyn and Katovich Hurley

10. Next-Generation HLA Sequencing using the 454 GS FLX System

Elizabeth A. Trachtenbergand Cherie L. Holcomb

11. Chimerism Testing by Quantitative PCR Using Indel Markers

Ketevan Gendzekhadze, Laima Gaidulis, and David Senitzer

12. Gene-specific PCR Typing of Killer Cell Immunoglobulin-like Receptors (KIR)

Raja Rajalingam and Elham Ashouri

13. Complement-dependent Cytotoxicity Crossmatch

Jeremy Ryan Pe a, Donna Fitzpatrick, and Susan L. Saidman

14. Lymphocyte Crossmatching by Flow Cytometry

Robert A. Bray

15. Solid Phase Assay Measuring C4d Deposition to Determine Complement Fixation by HLA Specific Antibodies

John D Smith and Marlene L Rose

16. C1q Assay for the Detection of Complement Fixing Antibody to HLA Antigens

Ge Chen and Dolly B. Tyan

17. Tetramer Staining for the Detection of HLA-specific B cells

Donna P. Lucas, Mary S. Leffell, and Andrea A. Zachary

18. A Flow Cytometric Crossmatch Test Using Endothelial Precursor Cells Isolated from Peripheral Blood

Annette M. Jackson, Donna P. Lucas, Jessica L. Badders

19. The Detection of Antibodies to the Angiotensin II-Type 1 Receptor in Transplantation

Duska Dragun

20. Detection of Antibodies to Self-antigens (K-alpha 1 Tubulin, Collagen I, II, IV and V Myosin and Vimentin) by Enzyme-Linked Immunosorbent Assay (ELISA)

Nayan J. Sarma, Venkataswarup Tiriveedhi, and T. Mohanakumar

21. Cylex ImmuKnow Cell Function Assay

Adriana Zeevi and John Lunz

22. Detection of Intracellular Cytokines

Nancy L. Reinsmoen and Chih-Hung Lai

23. Tolerogenic Dendritic Cells and Induction of T Suppresson Cells in Transplant Recipients

George Vlad and Nicole Suciu-Foca

24. Discovery and Customized Validation of Antibody Targets by Protein Arrays and Indirect ELISA

Tara K. Sigdel, and Minnie. M. Sarwal

25. RNA Purification and Expression Analysis using Microarrays and RNA Deep Sequencing

Steven R. Head, Tony Mondala, Terri Gelbart, and Phillip Ordoukhanian