Description

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Chapter 1 - Introduction
Overview of Single Molecule Force Spectroscopy Techniques
Experiments Using Single molecule AFM
Limitations of AFM-based single molecule force spectroscopy
Summary

Chapter 2- Correlation Force Spectroscopy
Correlation Force Spectroscopy: Rationale
Correlation Force Spectroscopy: Development
Laterally Offset Configuration
Vertically Offset Configuration
Analysis of Correlations between Two Cantilevers
Validation of Fluctuation-Dissipation Theorem for One Cantilever
Analysis of Thermal Fluctuations to obtain Correlations

Chapter 3- Dynamics of Single Molecules
Elastic Properties of Single Molecules: Worm-Like Chain and Freely-Jointed Chain Models
Hydrodynamics of Single Molecules: Dumbbell Model and Rouse Model
Internal Friction
Rouse with Internal Friction
Model of Linear Viscoelasticity of a Semiflexible Chain
Summary

Chapter 4- Microrheology with Correlation Force Spectroscopy
Existing Techniques of Rheometry
Experimental Methods
Comparison to Finite Element Analysis
Comparison to Simple Harmonic Oscillator Model
Summary

Chapter 5- Development of Colloidal Probe Correlation Force Spectroscopy: Case Study
Materials and Methods
Analysis
Results
Discussion
Summary

Chapter 6- Correlation Force Spectroscopy for Single Molecule Measurements
Effect of the Distance between Cantilever Tips
Harmonic Oscillator Modeling of Vertically Offset Correlation Force Spectroscopy
Summary

Chapter 7- Single Molecule Force Spectroscopy of Dextran
Materials and Methods
Results
Stretching a Dextran Molecule
Static Force-Elongation Mode
Dynamic Correlated Fluctuations Mode
Discussion
Summary

Chapter 8- Single Molecule Force Spectroscopy of Single-Stranded DNA
Rationale
Introduction Materials and Methods
Modal Analysis
Results
Discussion
Summary

Chapter 9- Summary
An Overview of Chapters
Future Work

Appendix I: Simple Harmonic Oscillator Model for Laterally Offset Correlation Force Spectroscopy

Appendix II: Simple Harmonic Oscillator Model for Vertically Offset Correlation Force Spectroscopy with Tethered Molecule

References

About the Author

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Milad Radiom received his PhD in Chemical Engineering at Virginia Tech in 2014, after MEng and BSc in Thermal and Fluids Engineering and Mechanical Engineering respectively from Nanyang Technological University and Amirkabir University of Technology. Thereafter, he was appointed as a postdoctoral research associate in Laboratory of Colloid and Surface Chemistry, University of Geneva. His research interests are physical chemistry of polymers, colloids and surfaces as related to single molecule force spectroscopy, micro-rheology and surface forces.